[maker-devel] gff pass through

[Reith, Michael]

Hi all,

I'm trying to use the gff pass through method to add the transcript/exon data generated by Cufflinks as EST information.  Maker works fine if I use a fasta file for the EST input, but dies (with "Bus error") if I try to use a gff3 file that I've built from the Cufflinks gtf output and validated with the on-line gff3 validator.  I was wondering:  1). if the gff pass through files referred to in the Maker tutorial (pass.tar.gz) might be available to test our set up (running on Solaris); 2). whether anyone has any bright ideas on why maker would be crashing only with the gff input.

Thanks,
Mike

-----------------------------------------------------
Michael Reith
Principal Research Officer
Functional Genomics Group Leader
NRC Institute for Marine Biosciences
1411 Oxford St.
Halifax, N.S.    B3H 3Z1
Canada

phone:  (902) 426-8276
fax:       (902) 426-9413
email:   michael.reith at nrc.ca
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