[maker-devel] Maker with Augustus
david.swarbreck
dswarbreck at stanford.edu
Thu Oct 15 12:29:14 MDT 2009
Hi,
I have a question relating to using Augustus as the predictor with
Maker. From looking at the documentation for augustus the hints files
detailing aligned features uses a non-standard GFF format. These include
the use of non SO compliant features such as exonpart (treated
differently to exon by augustus) and the inclusion of a source
identifier in col9 which has to be specified in the augustus config. In
addition in the standalone Augustus setting a bonus and penalty is
required for obeying a hint feature.
So my question is when using Augustus as the predictor in Maker is Maker
taking care of this for me or do i still need to define exonparts as
distinct from exon and provide bonus and penalty information. Is there a
particular GFF3 format which maker requires to define for example
alignments e.g can these use the match type (e.g. EST_match), can this
use the cigar format or should these features be described as exons.
Any help appreciated
david
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