[maker-devel] <MAKER Trac Server> #26: high-scoring blastx hit disappears

MAKER maker-devel at yandell-lab.org
Tue May 5 19:34:56 MDT 2009


#26: high-scoring blastx hit disappears
-------------------------------+--------------------------------------------
 Reporter:  sspao at ucdavis.edu  |       Owner:       
     Type:  defect             |      Status:  new  
 Priority:  major              |   Milestone:       
Component:  Code               |     Version:  0.001
 Keywords:                     |  
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 There's a high-scoring blastx hit that was present in a maker run against
 a protein database. But it disappears in a second maker run, when run
 against a larger protein database. Both protein databases contain the
 sequence (CAN65888). This is verifiable because there are intermediate
 files created and both maker runs contain the blastx file. maker_bopts.ctl
 options are the same for both maker runs.

 When comparing the GFF files from the two maker runs, there is a blastx
 and a protein2genome hit (~0.17Mb reverse direction) but in the other GFF
 file, those two hits are not there. GFF files and blastx files attached.

-- 
Ticket URL: <http://malachite.genetics.utah.edu/projects/maker/ticket/26>
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